Biotechnology-principles and processes practice · Mixed difficulty

Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

The tumour inducing capacity of Agrobacterium tumefaciens is located in large extrachromosomal plasmids called

In which of the following method, a probe is allowed to hybridise to its complementary DNA in the clone of cells?

The autonomously independent self-replicating extra nuclear DNA imparting certain factors to some bacterium is called

Which one of the following is correct explanation for autoradiography?

In gel electrophoresis, DNA moves towards _______, because DNA has ________ charge. Choose the option which correctly fills the given blanks

In cloning vector pBR322 the recognition site sal i is present in

Biolistic technique is used in

The taq polymerase enzyme is obtained from:

A single strand of nucleic acid tagged with a radioactive molecule is called

Which one is associated with downstream processing?

a) Separation b) Purification
c) DNA isolation d) Gene expression

Select the odd one w.r.t. downstream processing.

When restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites between the same two base of opposite strands, it produces

Consider the following statements and identify the correct statements
I. While using genes encoding antibiotic resistence as selectable markers, such genes are selected for which host is naturally resistant
II. Restriction site of PvuII is present in $\mathrm{amp}^\mathrm{R}$ gene
III. Ti plasmid is most widely used in gene transfer in all plants
IV. Retroviruses are directly used (unchanged) to deliver desirable genes into animal cells

The enzyme employed for amplification of DNA during PCR is commercially obtained from

Downstream process includes
I. Separation of the product from the reactor
II. Purification of the product
III. Formation of the product with suitable preservatives
IV. Quality control testing and clinical trials in case of drugs
Which of the statements given above are correct?

In genetic engineering restriction endonuclease enzymes are used to make/start

Select the incorrect match

Single-stranded DNA molecules that can bind to and be used to detect other DNA molecules are called

For transformation with recombinant DNA, the bacterial cells must first be made ‘competent’ which means

Restriction endonucleases are

Which of the following correct ascending order of the number of the hydrogen bond in different DNA which are given below:

(a) 5'-AATAAAGC-3';$\quad$ 3'-TTATTTCG-5'

(b) 5'-AATGCTGC-3';$\quad$ 3'-TTACGACG-5'

(c) 5'-ATGCTGAT-3';$\quad$ 3'-TACGACTA-5'

(d) 5'-GCATAGCC-3';$\quad$ 3'-CGTATCGG-5'

Which of the following statements is correct w.r.t. plasmid?

Palindromic sequence are?

Following enzymes / techniques are used in the process of recombinant DNA technology
A) EcoRI to cut the isolated genome
B) DNA ligase
C) Protease and ribonuclease for removal of proteins and RNA from DNA
D) Production of recombinant hosts
E) Lysozyme for isolation of the genetic material (DNA)
F) Gel electrophoresis for separation and isolation of DNA fragments
Mark the correct sequence of their use

The volume of bioreactors cannot be

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

Plasmid pBR322 has Pstl restriction enzyme site within gene $\mathrm{am}{\mathrm{p}^R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta -$galactoside production and the recombinant plasmid is inserted in an E.coli strain

How many copies of DNA will be formed from DNA after 4 cycles of PCR?

When a ${P^{BR}}\,\,322$ plasmid is treated with ${B{am}}\,\,\,HI$ to clone a foreign insert successfully, then the transformed colony would be resistant to $\underline {\mathrm{'A'}}$ and sensitive to $\underline {\mathrm{'B'}} .$ A and B respectively