Biotechnology-principles and processes practice · Medium

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

Which one of the following is used as vector for cloning genes into higher organisms?

Select the odd one w.r.t. type of ends obtained after restriction enzyme digestion

Gene amplification using primers can be done by

Cosmid is

The first restriction endonuclease isolated was

A restriction endonuclease breaks bond between the

How many DNA molecules are generated after completion of 4 PCR cycles from 1 DNA molecule?

Study the following statements regarding recombinant DNA technology and select the incorrect ones.
(i)Taq polymerase extends the primers using the nucleotides provide in the reaction.
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology.
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis.
(iv) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
(v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture.
(vi) Downstream processing is one of the steps of polymerase chain reaction.

Comparing asexual reproduction with sexual reproduction, which among the following statement is incorrect:

Which of the following statement is not true?
I. DNA being a hydrophilic molecule cannot pass through cell membranes
II. $Agrobacterium\mathrm{ }tumefaciens$ delivers a piece of DNA known as ‘Z-DNA’ in the Ti-plasmid which transforms normal plant cells into tumour cells to produce chemical against pathogens
III. Retrovirus, adenovirus, papillomavirus are also now used as cloning vectors in animal because of their ability to transform normal cells into cancerous cell.
IV. In genetic engineering, DNA from different sources are cut with the same restriction enzymes so that both DNA fragments have same kind of sticky ends
Choose the correct option

Enzyme that is used in PCR technology is

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

Analyze the statements given below regarding the thermal cycler and select the option that shows
incorrect statements.

I. Annealing is also called the hybridization of oligonucleotide (primer).

II. During extension, DNA polymerase polymerizes nucleotides in 5' $\to$ 3' direction.
III. Denaturation is a process of formation of H-bonds.

Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used

In which of the following method, a probe is allowed to hybridise to its complementary DNA in the clone of cells?

In gel electrophoresis, DNA moves towards _______, because DNA has ________ charge. Choose the option which correctly fills the given blanks

In cloning vector pBR322 the recognition site sal i is present in

Biolistic technique is used in

A single strand of nucleic acid tagged with a radioactive molecule is called

The enzyme employed for amplification of DNA during PCR is commercially obtained from

Select the incorrect match

Restriction endonucleases are

Plasmid pBR322 has Pstl restriction enzyme site within gene $\mathrm{am}{\mathrm{p}^R}$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta -$galactoside production and the recombinant plasmid is inserted in an E.coli strain

When a ${P^{BR}}\,\,322$ plasmid is treated with ${B{am}}\,\,\,HI$ to clone a foreign insert successfully, then the transformed colony would be resistant to $\underline {\mathrm{'A'}}$ and sensitive to $\underline {\mathrm{'B'}} .$ A and B respectively

Manipulation of DNA in genetic engineering become easy due to invention of

A plasmid

EcoRI cuts the DNA between bases ‘X’ and ‘Y’ only when the sequence 5’ – GAATTC – 3’ is present in the DNA. These two bases form how many $H_2$ bonds, respectively, with their complementary $N_2$ bases?

Assertion: A bacterial cell with no restriction enzymes will be easily infected and lysed by bacteriophages.
Reason: Restriciton enymes catalyse synthesis of protective coat around bacterial cell that prevents bacteriophage attack.

What is true for plasmid?