Biotechnology-principles and processes practice · Mixed difficulty

Assertion: Special methods are used for
transformation i.e., incorporation of recombinant
DNA into molecule.

Reason: DNA is a hydrophilic molecule.

Proteins are removed by treatment with

Enzyme isolated from Thermus aquaticus bacterium is used in

${\mathrm{T}_\mathrm{i}}$ plasmids used in genetic engineering is obtained from

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

Which one of the following is used as vector for cloning genes into higher organisms?

Select the odd one w.r.t. type of ends obtained after restriction enzyme digestion

Gene amplification using primers can be done by

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:

The polymerase enzyme used in PCR is

Cosmid is

Restriction endonucleases are most widely used in recombinant DNA technology. They are obtained from

The first restriction endonuclease isolated was

A restriction endonuclease breaks bond between the

Which of the following enzyme is not used for extraction of DNA from a bacterium?

How many DNA molecules are generated after completion of 4 PCR cycles from 1 DNA molecule?

Study the following statements regarding recombinant DNA technology and select the incorrect ones.
(i)Taq polymerase extends the primers using the nucleotides provide in the reaction.
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology.
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis.
(iv) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
(v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture.
(vi) Downstream processing is one of the steps of polymerase chain reaction.

Comparing asexual reproduction with sexual reproduction, which among the following statement is incorrect:

Restriction endonuclease cuts

Which of the following is used in genetic engineering?

Which of the following statement is not true?
I. DNA being a hydrophilic molecule cannot pass through cell membranes
II. $Agrobacterium\mathrm{ }tumefaciens$ delivers a piece of DNA known as ‘Z-DNA’ in the Ti-plasmid which transforms normal plant cells into tumour cells to produce chemical against pathogens
III. Retrovirus, adenovirus, papillomavirus are also now used as cloning vectors in animal because of their ability to transform normal cells into cancerous cell.
IV. In genetic engineering, DNA from different sources are cut with the same restriction enzymes so that both DNA fragments have same kind of sticky ends
Choose the correct option

Enzyme that is used in PCR technology is

In gene gun method used to introduce alien DNA into host cells, microparticles of ________ metal are used.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell
B. Cutting of DNA at specific location by restriction enzyme
C. Isolation of desired DNA fragment
D. Amplification of gene of interest using PCR
Choose the correct answer from the options given below:

Popularly used artificially  plasmid is?

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

In genetic engineering, where genes can be inserted from one organism into another or back into the original organism uses which of the following techniques?

Average number of plasmids per bacterial cell is?

Gene transfer is present in

Analyze the statements given below regarding the thermal cycler and select the option that shows
incorrect statements.

I. Annealing is also called the hybridization of oligonucleotide (primer).

II. During extension, DNA polymerase polymerizes nucleotides in 5' $\to$ 3' direction.
III. Denaturation is a process of formation of H-bonds.