Biotechnology and its applications practice · Mixed difficulty

The crops engineered for glyphosate are resistant/tolerant to

Assertion (A): Gene therapy is a collection of methods that allows correction of a gene defect diagnosed in a child or embryo.

Reason (R): It involves delivery of normal gene into the individual or embryo to take over the function of the non functional gene.

First hormone produced by recombinant DNA technology is

Given below are two statements.

Statement I: GMO $\to$ Foreign gene is introduced into the organism,
and is usually maintained as extra-chromosomal entity
or is integration into the genome of the organism.

Statement II: Hybrid $\to$ cross is done between total genomes of two
species or strain.

Choose the correct answer from the option given
below:

Which of the following pH conditions are required for action of Bt toxin?

Treatment of a genetic disorder by manipulating genes is called

Choose the incorrect statement

Breeding of crops with high levels of minerals, vitamins and proteins is called

Consider the following statements
I. Transgenic animals are more sensitive to the toxic substance than non-transgenic animals
II. Useful biological products can be produced by introducing into transgenic animals the portion of DNA which codes for a particular product
III. Brazzein is a protein produced by a west African plant, Pentadiplandra brazzeana which is approximately 2000 time as sweet as sugar
Which of the statements are given above are correct?

Assertion: Hirudin is a type of protein that
prevents blood clotting.

Reason: The gene that encodes hirudin is
transferred into Brassica napus, where hirudin
is accumulated in the seeds .

Which of the following companies started selling humulin in the year 1983?

Insulin has its action on GLUT4 and leads to uptake of glucose mainly in_______cells.

Flavr savr variety of tomato, remains fresh for a longer period than normal tomato due to

Some of the steps involved in the production of humulin are given below. Choose the correct sequence
I) Synthesis of gene (DNA) for human insulin artificially
II) Culturing recombinant E.coli in bioreactors
III) Purification of humulin.
IV) Insertion of human insulin gene into plasmid vector
V) Introduction of recombinant plasmid into E.coli.
VI) Extraction of recombinant gene product from E.coli.

Statement-I: Disarmed ‘Ti’ – plasmid of Agrobacterium tumifaciens is used to transfer desired genes into plant cells
Statement-II: Restriction endonucleases act on sugar phosphate back bone of DNA molecule.

Bt toxin gene are isolated from Bacillus thuringiensis and incorporated into crop plants making them insecticidal. The choice of genes depend upon

Assertion (A): Specific Bt toxin genes were isolated from Bacillus thuringiensis and incorporated into the several crop plants such as cotton.

Reason (R): The choice of genes depends upon the crop and the targeted pest, as most Bt toxins are not insect-group specific.

Which of the following transgenic human protein product has been used to treat emphysema?

Biopiracy is related to which of the following

Which of the following transgenic plant is used for the synthesis of hirudin ?

Assertion: Using biotechnology human insulin
can be produced into bacterial cells.

Reason: To produce human insulin the A, B and
C polypeptides of the human insulin are
produced in the bacterial cells, separately
extracted and combined by creating disulfide
bonds.

Consider the following statements
I. Specific $Bt$ toxin gene have been isolated from $Bacillus\mathrm{ }thuringiensis$
II.$Bt$ toxin is coded by a gene named $cry$
III. $Bt$ toxin protein exists as inactive protoxins
Which of the statements given above are correct?

Which of the following vector is commonly used in transfer of gene of interest into the crop plants

Assertion (A): Biotechnology is an alternative path that our understanding of genetics can show so that farmers may obtain maximum yield from their fields
Reason (R): For farmers in the developing world, agrochemicals are often too expensive, and further increases in yield with existing varieties are not possible using conventional breeding

Bt toxins encoded by genes $c r y I A c$ and $c r y l l A b$ are used to control

Which of the following diagnostic technique is based on antigen antibody reaction?

ELISA is based on the principle of?

Detection of mutation in genes in suspected cancer patients can be done by

For an effective treatment of a disease:

Identify the mis-match