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NEET UG BIOLOGY MCQ: BIOTECHNOLOGY-PRINCIPLES AND PROCESSES
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Arrange the following in the descending order of their number in restriction site of E.coRI
I. Number of hydrogen bonds broken
II. Number of covalent bonds broken
III. Total number of hydrogen bonds
IV. Number of hydrogen bonds left intact after treating with enzyme
V. Number of unpaired nucleotides left at each end of the fragment
I. Number of hydrogen bonds broken
II. Number of covalent bonds broken
III. Total number of hydrogen bonds
IV. Number of hydrogen bonds left intact after treating with enzyme
V. Number of unpaired nucleotides left at each end of the fragment
View answer and explanation
Correct option: C
III, I, IV, V, II
Option C is correct. The correct order is III > I > IV > V > II. The total number of hydrogen bonds is the highest, followed by the number broken, then those remaining intact, then the unpaired nucleotides, and finally the covalent bonds broken (which is the lowest).
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transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?