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NEET UG BIOLOGY MCQ: BIOTECHNOLOGY-PRINCIPLES AND PROCESSES
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Assertion: A single stranded DNA tagged with radioactive molecule is allowed to hydridise followed by autoradiography, the clone having the mutated gene does not appear.
Reason: The clone having the mutated gene does not appear on the photographic film as the probe used will not have complementarity with the mutated gene.
Reason: The clone having the mutated gene does not appear on the photographic film as the probe used will not have complementarity with the mutated gene.
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Correct option: A
Both assertion and reason are true and reason is the correct explanation of assertion
Option A is correct. If the probe is complementary to the normal gene but not the mutated gene, it will not hybridize to the mutated gene, and no signal will appear on the autoradiograph.
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Assertion: Special methods are used for
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?