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NEET UG BIOLOGY MCQ: BIOTECHNOLOGY-PRINCIPLES AND PROCESSES
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When a plasmid is treated with to clone a foreign insert successfully, then the transformed colony would be resistant to and sensitive to A and B respectively
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Correct option: A
Ampicillin and Tetracyclin
Option A is correct. BamHI cuts within the tetracycline resistance gene. Therefore, successful insertion of a foreign gene at this site will disrupt tetracycline resistance, leaving the transformed colony resistant to ampicillin (due to the intact ampicillin resistance gene) but sensitive to tetracycline.
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Assertion: Special methods are used for
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?