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NEET UG BIOLOGY MCQ: BIOTECHNOLOGY-PRINCIPLES AND PROCESSES
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Plasmid pBR322 has Pstl restriction enzyme site within gene that confers ampicillin resistance. If this enzyme is used for inserting a gene for galactoside production and the recombinant plasmid is inserted in an E.coli strain
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Correct option: C
It will not be able to confer ampicillin resistance to the host cell
Option C is correct. Insertion of the gene for -galactosidase into the PstI site within the ampicillin resistance gene will disrupt the ampicillin resistance gene, making the host cell unable to confer ampicillin resistance.
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Assertion: Special methods are used for
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?