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NEET UG BIOLOGY MCQ: BIOTECHNOLOGY-PRINCIPLES AND PROCESSES
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Following enzymes / techniques are used in the process of recombinant DNA technology
A) EcoRI to cut the isolated genome
B) DNA ligase
C) Protease and ribonuclease for removal of proteins and RNA from DNA
D) Production of recombinant hosts
E) Lysozyme for isolation of the genetic material (DNA)
F) Gel electrophoresis for separation and isolation of DNA fragments
Mark the correct sequence of their use
A) EcoRI to cut the isolated genome
B) DNA ligase
C) Protease and ribonuclease for removal of proteins and RNA from DNA
D) Production of recombinant hosts
E) Lysozyme for isolation of the genetic material (DNA)
F) Gel electrophoresis for separation and isolation of DNA fragments
Mark the correct sequence of their use
View answer and explanation
Correct option: C
E,C,A,F,B,D
Option C (E,C,A,F,B,D) is correct as it represents the logical sequence of steps in recombinant DNA technology.
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Assertion: Special methods are used for
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?
transformation i.e., incorporation of recombinant
DNA into molecule.
Reason: DNA is a hydrophilic molecule. Proteins are removed by treatment with Enzyme isolated from Thermus aquaticus bacterium is used in plasmids used in genetic engineering is obtained from Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain? Which one of the following is used as vector for cloning genes into higher organisms?